Stanford Hospital & Clinics and Lucile Packard Children’s Hospital
Test Name : Anti-Phospholipid Antibody Panel Order Code : APHSA2
Note: Test performed by Special Coagulation Lab
Synonyms: Anti-Phospholipid Antibodies
Specimen Type: Platelet-Free Plasma
Container Type: 2 Light Blue-top tubes (Sodium Citrate), and 2 Red-top tube (Plain).
Required Volume: 2 full blue top tubes (4.5 mL blood each) and 5 mL in red top tubes
Minimum Volume (Pediatric): 1 Full blue top tube (4.5mL blood) and 2mL in red top tube
Methodology: See methodology for individual tests (LUPUS, ACA. B2GP1, APSPT and DRVVTP) in this panel.
Components: Anticardiolipin Antibody, IgG and IgM; Lupus Anticoagulant;Beta-2 Glycoprotein 1; Dilute Russell Viper Venom; Phosphatidylserine/Prothrombin Antibodies
Standard Run Times: Mon-Fri, batched, urgent test upon request
Turnaround Time: 24 hours/next day
Special Handling: Blue top tube: Within 1 hour of collection, centrifuge tubes as required to obtain platelet poor plasma. The plasma is transferred to a plastic tube to be spun a second time to remove platelets and obtain platelet-free plasma. Aliquot platelet free plasma into plastic tubes (minimum 4 aliquots). Red top tube: Allow the red top tube to clot completely and retract from the side of the tube (approximately 1 hour), centrifuge, separate serum, transfer serum to screw-top tubes (2 aliquots) and freeze. Label tubes "Serum".Freeze all the aliquots immediately. Transport Frozen. Label tubes "Plasma" or "Serum" respectively.
CPT Codes: See each individual tests
Causes For Rejection: Clotted blue-top tube, gross hemolysis, improperly filled tubes, specimens received thawed.
Department: Coagulation