Stanford Hospital & Clinics and Lucile Packard Children’s Hospital
RESULT
Test Name : T and B Lymphocyte Subsets, Whole Blood Order Code : TBLYM
Note: Only dual CD8 and CD3-positive lymphocytes are enumerated as suppressor cells, eliminating T cytotoxic or NK cells, which may have weak surface suppressor antigen without pan-T CD3 expression. Absolute counts calculated using electronic impedance WBC and % lymphocytes.
Synonyms: Lymphocyte Phenotyping Blood
Specimen Type: Whole Blood
Container Type: Lavender-top tube (EDTA)
Required Volume: 4 mL
Minimum Volume (Pediatric): 0.5 mL
Methodology: Flow Cytometer, Automated Hematology Analyzer
Components: WBC count, lymphocyte % and absolute count, CD3 % and absolute count, CD19 % and absolute count, CD4 % and absolute count, CD8 % and absolute count, CD3 neg/CD16+56 (NK) % and absolute count, CD4/CD8 ratio
Standard Run Times: Daily
Turnaround Time: 24-48 hours
Special Handling: Transport at Room Temperature, to be received within 24 hours of collection.
CPT Codes: 86355, 86357, 86359, 86360
LPCH EPIC Code: LAB3188
SHC EPIC Code: LABTBLYM
Causes For Rejection: Severely hemolyzed specimen, clotted specimen, improperly labeled specimen
Department: Flow Cytometry