DEPARTMENT: FLOW CYTOMETRY / SPECIAL HEMATOLOGY
DIRECTOR: Daniel Arber, M.D.
DIRECTOR: Susan Atwater, M.D.
NEW TEST: CD34 Panel, Blood, Bone Marrow and Fluid
Test Description and Clinical Indication: CD34 is a transmembrane glycoprotein found on human myeloid and lymphoid progenitor cells, as well as endothelial progenitor cells. CD34+ progenitor cells are used in stem cell transplantation to repopulate the bone marrow after myeloablative conditioning. Prior to transplantation, patients are prepared for stem cell harvesting using G-CSF mobilization, and then peripheral leukocytes are collected by apheresis. Stem cell enumeration ensures that CD34+ stem cells are present in sufficient numbers to repopulate the bone marrow following storage.
This assay enumerates CD34+ progenitor cells by flow cytometry using a PE-conjugated class III CD34 reagent antibody, with data analysis using the ISHAGE sequential gating strategy. This gating strategy selects the population of interest while minimizing interference from debris and nonspecific CD34 reagent binding to non-progenitor cells.
CD34 Panel, Blood |
Order Code: CD34 |
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Specimen Type: |
Whole Blood |
Container Type: |
Lavender-top tube (EDTA) |
Required Volume: |
3 mL |
Minimum Volume (Pediatric): |
1 mL |
Special Handling: |
Room Temperature, received within 24 hours of collection. |
Methodology: |
Flow Cytometry, gating ISHAGE |
Components: |
CD34 % and absolute count |
TAT: |
24 hours/next day |
CPT Codes: |
86367 |
Report: |
Reported as CD34% and absolute count |
CD34 Panel, Bone Marrow |
Order Code: BMCD34 |
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Specimen Type: |
Bone Marrow |
Container Type: |
Lavender-top tube (EDTA) |
Required Volume: |
1 mL |
Minimum Volume (Pediatric): |
1 mL |
Special Handling: |
Room Temperature, received within 24 hours of collection. |
Methodology: |
Flow cytometry, gating ISHAGE |
Components: |
CD34 % and absolute count |
TAT: |
24 hours/next day |
CPT Codes: |
86367 |
Report: |
Reported as CD34% and absolute count |
CD34 Panel, Fluid |
Order Code: FCD34 |
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|
Specimen Type: |
Apheresis Fluid |
Container Type: |
Sterile tube or container |
Required Volume: |
1 mL |
Minimum Volume (Pediatric): |
1 mL |
Special Handling: |
Room Temperature, received within 24 hours of collection. |
Methodology: |
Flow Cytometry, gating ISHAGE |
Components: |
CD34 % and absolute count |
TAT: |
24 hours/next day |
CPT Codes: |
86367 |
Report: |
Reported as CD34% and absolute count |
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